Design of the DNA dodecahedron
To generate the 20 trisoligonucleotides for the self-assembly of the DNA dodecahedron 30 independent 15 bp DNA sequences with narrow melting points were calculated using the program SequenceGenerator of Banzhaf et al.[45] The 30 sequences were assigned to the 30 edges of the dodecahedron (Table 2). As a result, a set of twenty trisoligonucleotides was determined in such a way that each trisoligonucleotide represents a vertex of the dodecahedron and the complementary "arms" of neighboring trisoligonucleotides form duplexes, representing the edges of the dodecahedron.
Table 2 Trisoligonucleotide sequences assigned to each of the 20 vertices of the dodecahedron, connectivities as depicted in the Schlegel representation (right). Each 3' end is attached to trislinker 1 via a phosphodiester linkage.
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Synthesis of the trisoligonucleotides and self-assembly of the
DNA dodecahedron
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Characterization of the DNA dodecahedron
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Addressable functionalization of the nano structure
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Trisoligonucleotides with a C3h-symmetric
linker