The most recent application of model-based evolutionary optimization for vesicle properties is in the area of optimization for drug delivery [1]. Here, vesicle composition is optimized to maximize the carrying capacity of a target drug (amphotericin B), by use of a fluorescent assay.
The experimental variables explored were concentrations of different amphiphile types, pH buffers, and salts.
Again we see optimization after just 3 generations. Though the maximum peak is roughly equivalent for these generations, the mean fitness increases consistently.